Donnerstag, 15. Dezember 2016

MIQE & qPCR iBook -- What's New in Version 2.5

How to apply the MIQE guidelines - a visual, interactive and practical qPCR guide

2nd edition (version 2.5 published 12th Dec 2016)
Editors:  Afif M. Abdel Nour & Michael W. Pfaffl
ISBN 9783000488061
Free download via iTunes

What's New in Version 2.5

The present second edition of the MIQE & qPCR book should help to spread this MIQE idea even further in any laboratory worldwide and beyond in the scientists’ workflow and minds. It should clearly show how to apply the guidelines and serve as a handy, visual, interactive and practical guide. For now in the first year after publication of the first edition we could count more than 1200 downloads of the MIQE & qPCR book from more than 30 countries. Our goal for the second edition is to update the existing content by new chapters, and to improve this fancy interactive tool, interfacing scientific publications with educating pictures, videos and scientific talks. We implemented multiple new chapters, describing the significance of the reverse transcription reaction, why qPCR assay validation is so important for high sensitivity and good reproducibility, and one reviewing chapter about the necessity of performing quality control at all levels in the qPCR workflow.

Mittwoch, 14. Dezember 2016

qPCR dPCR NGS 2017 -- Call for POSTER & TALK Abstracts

Scientific Symposium Sessions:
- Liquid Biopsy & Circulating Nucleic Acids
- Advanced Molecular Diagnostics
- Integrative Big Data Analysis
- Biomarker Signatures
- Digital PCR
- Non-coding RNAs -- microRNA, isomiRs, small RNAs, long non-coding RNAs
- MicroGenomics & Single-Cells Diagnostics
- Next Generation Sequencing (NGS)
- Molecular Diagnostics in Life Science
- MIQE & QM & Standardisation Strategies in Molecular Diagnostics

Register and submit your scientific contribution (talk or poster abstracts) =>

Valid biomarker signatures from liquid biopsies - how to standardize next generation sequencing

Dominik Buschmann1,2, Benedikt Kirchner1,3, Michael W. Pfaffl1
1 Department of Animal Physiology and Immunology, TUM School of Life Sciences Weihenstephan, Freising, Germany
2 Institute of Human Genetics, University Hospital, Ludwig-Maximilians-University Munich, Munich, Germany
3 Dr. von Hauner Children's Hospital, Ludwig-Maximilians-University Munich, Munich, Germany

The advent of Next-Generation Sequencing (NGS) techniques has revolutionized transcriptomics research and opened numerous avenues for scientific and clinical applications. While reverse transcriptase quantitative real-time PCR (RT-qPCR) is still considered the gold standard of gene expression analysis, its high throughput, single-nucleotide resolution and ever-plummeting costs have made NGS an intriguing and increasingly accessible alternative to this classical method. In addition to mere transcript quantification, RNA-Seq offers exciting new insights such as the discovery of novel transcripts and detection of alternative splice variants or chimeric transcripts. While DNA sequencing yields fascinating discoveries about the genomic makeup of target tissues, RNA-Seq might hold even more potential for biomarker research and drug discovery. 

... more about Liquid Biopsies

Dienstag, 22. November 2016

MIQE guideline was cited 5.000 times

The qPCR and dPCR MIQE guidelines – A success story!
The MIQE guidelines and the resulting scientific validity will be supported by more and more researchers, biological journals, academic and commercial institutions. Today late November 2016 we count 5000 citations for the MIQE guideline applied in qPCR and 185 citations for the digital PCR (dPCR) MIQE guideline (measured by Google Scholar). Hence the qPCR and dPCR MIQE guidelines are a worldwide full success story which will be driven forward by the scientific community.

Donnerstag, 17. November 2016

Accelerating your qPCR data analysis | qbase+

Your benefits using qbase+

Save time
  • The analysis wizard guides you through different steps to perfom reliable analysis of your real-time PCR data
  • Excluding bad data is easy 
  • Effortless scaling for larger experiments
  • Stop wondering where to find your data, qbase+ stores all of it in a structured way!
More reliable results
  • qbase+ is based on peer-reviewed quantification models for PCR efficiency correction, error propagation, inter-run calibration and statistics (Hellemans et al., Genome Biology, 2007). 
  • Extensive quality control which is (often) neglected in Excel-templates
  • Reduced risk of making mistakes in comparision to Excel-templates
MIQE & RDML compliancy
  • qbase+ helps applying rigorous MIQE compliant procedures and guides the experimenter to highest quality results. The analyzed and annotated experiments can easily be exported to RDML 1.2 files which can be used as supplemental data for publication.