Dienstag, 12. Mai 2015

MIQE & qPCR iBook (1st edition)

MIQE & qPCR iBook

How to apply the MIQE guidelines - a visual, interactive and practical qPCR guide

1st edition by Afif M. Abdel Nour & Michael W. Pfaffl
ISBN 9783000488061
Free download on iTunes

Throughout the past 30 years Polymerase Chain Reaction (PCR) has proven to be the most powerful technique in a scientist toolbox. Only few techniques had a comparable success story like PCR. This iBook will guide you in better practicing in your laboratory thanks to the MIQE guideline.

More qPCR books and iBooks  http://qPCRbooks.gene-quantification.info

Montag, 11. Mai 2015

Advances in Oncology & Symposium on Molecular Medicine

20th World Congress on Advances in Oncology &
18th International Symposium on Molecular Medicine
October 8-10, 2015
Metropolitan Hotel, Athens, Greece

Pre-Congress qPCVR Workshops
in cooperation with the TATAA Biocenter, Sweden
6-7 October 2015
‘MIQE - How to get good-quality control in qPCR’ (2-day workshop by Dr. Kristina Lind)
‘Discovery and Validation of Expression Biomarkers — Experimental Design and Data Analysis’ (2-day workshop by Prof. Mikael Kubista)

Industrial Exhibition
In parallel to the scientific symposium of 2015, an Industrial Exhibition will take place, at which international companies, leaders in the fields of Oncology and Molecular Medicine, will present their latest technologies and services in clinical diagnostics. If you are interested in joining the exhibition

Contact Details

Metropolitan Hotel, Athens, Greece

Dienstag, 31. März 2015

First impressions from qPCR & NGS 2015 Event

First impressions from qPCR & NGS 2015 Event
23rd - 27th March 2015
480 participants from 38 countries

Talk Agenda -- http://AgendaPDF.qPCR-NGS-2015.net
Poster Session -- http://Posters.qPCR-NGS-2015.net

Montag, 16. März 2015

qPCR, dPCR, NGS – A journey

qPCR, dPCR, NGS – A journey
Jim F. Huggett, Justin O’Grady, Stephen Bustin
Biomolecular Detection and Quantification, available online 15 January 2015

Scientific conferences fulfill many roles, but one of the most important ones is that they help shape the direction in which a scientific discipline grows by promoting person-to-person exchanges of information, ideas and constructive criticisms between scientists from different backgrounds. This interaction also helps to identify areas of controversy and promotes efforts to address and, it is hoped, resolve them. This year is the 30th anniversary of the publication of the first practical description of the polymerase chain reaction [1], arguably one of the simplest and the most widely used molecular technology. It also sees the 7th instalment of the Freising PCR meetings http://www.qPCR-NGS-2015.net, which are the longest established, continuous and most influential conferences in this field and have provided a looking glass for conceptual and technical innovation as well as practical applications of PCR-associated methods.

Donnerstag, 12. März 2015

How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments

How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments

David Svec, Ales Tichopad, Vendula Novosadova, Michael W. Pfaffl, Mikael Kubista
iomolecular Detection and Quantification (March 2015)

We have examined the imprecision in the estimation of PCR efficiency by means of standard curves based on strategic experimental design with large number of technical replicates. In particular, how robust this estimation is in terms of a commonly varying factors: the instrument used, the number of technical replicates performed and the effect of the volume transferred throughout the dilution series. We used six different qPCR instruments, we performed 1–16 qPCR replicates per concentration and we tested 2–10 μl volume of analyte transferred, respectively. We find that the estimated PCR efficiency varies  significantly across different instruments. Using a Monte Carlo approach, we find the uncertainty in the PCR efficiency estimation may be as large as 42.5% (95% CI) if standard curve with only one qPCR replicate is used in 16 different plates. Based on our investigation we propose recommendations for the precise estimation of PCR efficiency: (1) one robust standard curve with at least 3–4 qPCR replicates at each concentration shall be generated, (2) the efficiency is instrument dependent, but reproducibly stable on one platform, and (3) using a larger volume when constructing serial dilution series reduces sampling error and enables calibration across a wider dynamic range.