Mittwoch, 31. Oktober 2018

Join the PCR & NGS Workshop @ 9th Gene Quantification Event

Join the PCR & NGS Workshop @ 9th Gene Quantification Event 
Event & Workshop announcement --

#GQ2019 PCR & NGS Workshop Topics:
  1. Basic real-time qPCR Application Workshop (2-days)  hosted by TATAA Biocenter
  2. Analysis of Gene Expression Data - qPCR, RNA-Seq, Microarray, Nanostring; From pre-processing and quality control to normalization, statistical analysis and modelling (2-days)  by TATAA
  3. Digital-PCR workshop (2 days)  hosted by Bio-Rad
  4. NGS – Library Construction and Quality Control (2-days)  hosted by TATAA
  5. Next-Generation Sequencing Data Analysis -- How to Call Genomic Variations and Uncover Their Effects (2-days)  hosted by ecSeq

Sonntag, 8. Juli 2018

9th Gene Quantification Event -- qPCR dPCR & NGS 2019

9th Gene Quantification Event -- qPCR dPCR & NGS 2019
Symposium & Industrial Exhibition & Application Workshops
Main topics: Next Generation Biomarkers -- Liquid Biopsy, Multi-Omics, MicroGenomics
18-22 March 2019, in Freising-Weihenstephan,
School of Life Sciences, Technical University of Munich, Weihenstephan, Germany
Download Event Announcement

Impressions from a great GQ2017 symposium

Please register and submit your scientific contribution via our online registration tool

Symposium Sessions

• Main topic:   Next-Generation Biomarkers
• Main topic:   Liquid Biopsy & Circulating Nucleic Acids
• Main topic:   Multi-Omics & Integrative Big Data Analysis
• Main topic:   MicroGenomics & Single-Cells Diagnostics
• ISEV Satellite Session – EV & Exosome Diagnostic Biomarkers
• Digital PCR
• Non-coding RNAs -- microRNA, isomiRs, small RNAs, long non-coding RNAs
• Next-Generation Sequencing (NGS)
• qPCR & NGS data analysis
• Advanced Molecular Diagnostics in Life Science
• MIQE & QM & Standardisation Strategies in Molecular Diagnostics
• Technical Lunch-Time Seminars

Submission deadline is 15th January 2019

Mittwoch, 4. April 2018

Gene Quantification Event Calendar 2018

NGS, qPCR and dPCR Event in Sydney, Australia

Confirmed Speakers Include:
• Prof Carl Wittwer - Professor of Pathology at the University of Utah Medical School and adjunct professor of BioMedical Engineering at the University of Utah. Inventor of SYBR Green qPCR and HRM
• Dr Jan Ruijter - Inventor of LinReg PCR
• ​Prof Michael W. Pfaffl - Life Science Center Weihenstephan, Technical University, Munich, Germany. Inventor of REST Analysis

Mini-symposium on digital PCR
May 15, 2018  09:30 - 16:30
Auditorium E (entrance 32),  Campus UZ Ghent,  Corneel Heymanslaan 10,  9000 Ghent,  Belgium

Keynote speaker:   Dr. J. Huggett,  LGC and UCL, UK
Dr Huggett read genetics in Liverpool University followed by a PhD in Cardiff University where he studied the transcription in bone disease. Particularly interested in the  application of advanced molecular methods to clinical scenarios he moved to UCL in 2002 to take up a senior research fellow position with Professor Alimuddin Zumla. Here he investigated diagnostic approaches to infectious diseases in developing world settings before moving to LGC in 2009, while retaining an honorary lectureship at UCL, which he still holds. At LGC, which is the UK’s designated national measurement institute role for chemical and bio-analytical measurement, he has led a large number of molecular diagnostics, genomics and nucleic acid research projects. This work focuses on high accuracy measurement as well as strengthening the traceability of measurements that underpin legislation, regulation and standardisation. In 2016 Dr Huggett joined the University of Surrey as a Senior Lecturer and holds a joint appointment with LGC.

Single Cell Europe 2018

September 19 - 21, 2018, Prague, Czech Republic

Single cell Europe 2018 is the first specialized meeting for single cell analysis in Central Europe and will be held in BIOCEV (Biotechnology and Biomedicine Center of the Academy of Science and Charles University in Vestec), a modern research and development center within easy reach of the beautiful historical city Prague in the Czech Republic.
You will hear presentations from world leading scientists from various areas in the single cell field, innovative companies exhibiting latest products and technologies for single cell analysis and you can join hands-on training on single cell expression profiling getting opportunity to test state-of-the-art technologies. It is also excellent opportunity for networking and finding collaborators in this rapidly developing field.

... and more events here

Mittwoch, 7. März 2018

GenEx (version 6.1) – A perfect and MIQE compliant tool for any qPCR data analysis

GenEx – the ultimate tool for qPCR data analysis
Mikael Kubista & Amin Forootan
1 TATAA Biocenter AB, Göteborg, Sweden;  2 MultiD Analyses AB, Göteborg, Sweden

Download a free GenEx trial version @

GenEx was the first commercial software developed for qPCR, and has since then become market leader with few thousands of users and several thousands of publications using GenEx. Sup- ported by all leading qPCR instrument manufacturers GenEx has batch import and corrects for inter-run variation. During pre- processing missing values are imputed, outliers are handled, and data are optimally normalized. Absolute quantification includes determining limit of detection, limit of quantification and dynamic range. All parameters are determined with confidence ranges in line with CLSI, CEN and ISO guidelines. Expression data are ana- lyzed with univariate methods including t-test, non-parametric methods and various ANOVAs with appropriate corrections for multiple testing ambiguity or multivariate methods including hierarchical clustering, Principal Component Analysis (PCA), Self- Organizing Map (SOM), Artificial Neural Network (ANN), Support Vector Machines (SVM), and many more. All analyses are easily per- formed with few clicks with the mouse using intuitive workflows that generate results that are readily interpreted in a biologi- cal context. At this lunch seminar, we will present GenEx and exemplify how easily multiple run files are in imported, measured data pre-processed, and how data are interactively analyzed using dynamic PCA to reveal key expression pathways and correlations across samples. All participants will receive FREE GenEx license for limited time.

The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. SA Bustin et al., Clinical Chemistry 55 (4), 611-622

Donnerstag, 22. Februar 2018

Australian NGS, dPCR & qPCR Symposium 2018 -- 24-25 May in Sydney

​Confirmed Speakers Include:
  • Prof Carl Wittwer - Professor of Pathology at the University of Utah Medical School and adjunct professor of BioMedical Engineering at the University of Utah. Inventor of SYBR Green qPCR and HRM
  • Dr Jan Ruijter - Inventor of LinReg PCR
  • Prof Michael W. Pfaffl - Life Science Center Weihenstephan, Technical University, Munich, Germany. Inventor of REST Analysis

​Enjoy the Taronga Centre's boutique dining and million dollar views,
while listening to the latest developments in:
  • qPCR and NGS
  • qPCR Inhibition
  • qPCR data analysis
  • Extreme qPCR
  • HSM (High Speed Melting Analysis)
  • HRM (High Resolution Melt Analysis)
  • Highly multiplex qPCR tests
  • Multiplex-Tandem PCR
  • Coupling PCR to single-molecule digital counting
  • Digital PCR
  • Efficiency determination (standard curve vs linear regression analysis)
  • 3 base qPCR