Call for Abstracts - qPCR 2010 Event

qPCR 2010 Event – The ongoing evolution of qPCR

http://www.qpcr2010-vienna.net

Download - Call for Abstracts

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BioEPS GmbH is organizing the qPCR 2010 Event taking place April 7th – 9th, 2010 in Vienna, Austria. Scientists from all around the world will come to exchange ideas, share experiences, and discuss the exciting future of the perhaps most powerful analytical technology ever developed in the life sciences area – the quantitative real-time polymerase chain reaction (qPCR). We are proud to present 40 scientific talks from invited international scientists and diagnostic companies in the qPCR field who will show their latest research findings and newest technologies. Focus of the event will be - The ongoing evolution of qPCR.

The scientific organization is managed by international well-known scientists in the field of qPCR:

Stephen Bustin Prof. of Molecular Science, School of Medicine, London, UK
Mikael Kubista Prof. of Biotechnology, TATAA Biocenter, Sweden
Christine Mannhalter Prof. for Molecular Diagnostics in Clinical Chemistry, Med. University Vienna, Austria
Jo Vandesompele Prof. at the Center of Medical Genetics, University of Ghent, Belgium
Michael W. Pfaffl Senior Scientist and Reader in Physiology, TUM, Weihenstephan, Germany

Using qPCR the amount of target nucleic acid in a complex sample can be determined with high precision, great accuracy, excellent specificity and the ultimate sensitivity of detecting a single molecule. The technique has revolutionized all molecular sciences and diagnostic applications. Conference presentations will include MIQE guidelines & QM strategies in qPCR, high performance nucleic acid extraction, single-cell applications, Epigenetics & High-Resolution-Melt analysis, circulating nucleic acids, and application involving RNAi and microRNA.
Further developments of qPCR technology will be presented include improved instrumentation, miniaturization, high throughput platforms, cost efficacy, validity, flexibility, quality assessment and reliable Cq calculations, expression data comparisons, and interpretation.
Today there is no field in the life sciences research, molecular biology and diagnostics areas that has not introduced qPCR technology for nucleic acid analysis. The combination with reverse transcription enables determination of mRNA, microRNA and widely opens the window for “Transcriptomics” – the first step of quantitative “Gene Expression Profiling” and “Functional Genomics”.
An Industrial Exhibition will take place parallel to the symposium, with 32 leading biotechnology companies presenting their latest developments, including real-time PCR cyclers, NA extraction robots, consumables, new fluorescence dyes, NA detection and amplification chemistries, as well as real-time PCR data analysis software.

For more information about the qPCR 2010 event contact Dr. Martina Reiter martina.reiter@bioeps.com

Hope to meet you in April in Vienna!

Michael Pfaffl
scientific coordinator

NEW BOOK - The PCR Revolution: Basic Technologies and Applications

The PCR Revolution: Basic Technologies and Applications
edited by Stephen A. Bustin

The invention of the polymerase chain reaction (PCR) won the Nobel Prize in Chemistry in 1994 and remains one of the most important scientific discoveries of the twentieth century. More than 50,000 researchers in the United States use PCR replication technology, and yet a book has not been published on the subject in more than ten years. In this book, Dr. Stephen A. Bustin, a world-renowned PCR expert, examines in detail the latest innovations and the overall impact of PCR on many areas of molecular research. The book contains personal reflections, opinions, and comments by leading authorities on the many applications of the PCR and how this technology has revolutionized their respective areas of interest. This book conveys the ways in which PCR has overcome many obstacles in life science and clinical research and also charts the PCR's development from time-consuming, low throughput, non-quantitative procedure to today's rapid, high throughput, quantitative super method.

Inside This Book - Browse Sample Pages:
Table of Contents First Pages Index

International qPCR 2010 Symposium & Exhibition in Vienna

International qPCR 2010 Symposium & Exhibition in Vienna
7th – 9th April 2010
The focus of the qPCR 2010 Event will be “The ongoing evolution of qPCR”

Our online registration and abstract submission tool - CONFTOOL - is available now => http://registration.qpcr2010-vienna.net

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qPCR 2010 in Vienna

International qPCR 2010 Symposium and Exhibition in Vienna 7th – 9th April 2010.
The focus of the qPCR 2010 Event will be “The ongoing evolution of qPCR” representing all new and emerging techniques, applications and data analysis methods. MIQE, HRM, microRNA, CNV, single-cell qPCR, digital PCR, and analysis of circulating nucleic acids will be in the focus of the conference => http://www.qpcr2010-vienna.net/

qPCR Symposium topic - “The ongoing evolution of qPCR”

The symposium focus will be on 40 lectures presented by international recognized experts in their application fields. The emphasis will be on unbiased, didactic information exchange. One third of the talks will be presented by invited speakers, one third of the speakers will be selected from the submitted abstracts and one third will be qPCR company representatives with their newest qPCR technologies.

The focus of the qPCR 2010 Event will be “The ongoing evolution of qPCR” representing all new and emerging techniques, applications and data analysis methods:

Symposium sessions & preliminary titles

1. MIQE and QM strategies in qPCR
   Prof. Stephen Bustin, “The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments”
2. High throughput quantitative PCR – digital PCR
   Prof. Mikael Kubista, “Digital PCR and intracellular expression profiling”
   Dr. Philip Day, “High throughput droplet PCR”
   Dr. Ken Livak, "title to be announced"
3. HRM – High Resolution Melting - Epigenetics
   Prof. Carl Wittwer, "High Resolution Melting Analysis"
   Prof. Claudio Orlando, “High Resolution Melting Analysis in Cancer Diagnosis”
4. Circulating nucleic acids
   Dr. Pamela Pinzani, “Cell free circulating DNA”
   Dr. Alfred Schöller, „Targeting the human urine RNAome for tumor diagnostics by qPCR”
   Dr. Jim Huggett, "Diagnostic tools for measuring cell free nucleic acids. What can we expect from the next decade?”
5. Single-cell qPCR
   Dr. Michael W. Pfaffl, “Quantitative expression analysis after pre-amp in single WBCs”
   Dr. Anders Stahlberg, “Single-cell gene expression
6. RNAi - microRNA - siRNA Applications – miRNA normalisation
   Prof. Jo Vandesompele, “MicroRNA and mRNA gene expression normalization”
   Dr. Mirco Castoldi, "Expression profiling of microRNA by quantitative real time PCR, what is available and where to go from there"
7. qPCR BioStatistics & BioInformatics
   Dr. Ales Tichopad, “Statistical aspects of quantitative PCR experiment design and qPCR data analysis”
   Dr. Jan Hellemans, “Accurate and objective copy number profiling using real-time quantitative PCR”
   Dr. Anders Bergkvist, “Expression profiling - clusters of possibilities”

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Contact information:

Dr. Martina Reiter

BioEPS GmbH
Lise Meitner Strasse 30
85354 Freising Weihenstephan
Germany
qPCR2010@BioEPS.com

Standardization of qPCR and RT-qPCR

PCR Technology Review: Standardization of qPCR and RT-qPCR
New Guidelines Seek to Promote Accurate Interpretation of Data and Reliable Results
by Stephen A. Bustin, Jo Vandesompele, Michael W. Pfaffl

The perceived ease of use of real-time quantitative PCR (qPCR) and reverse transcription PCR (RT-qPCR) technology has revolutionized life science research. Its effectiveness at amplification and quantification of low levels of nucleic acids has driven the emergence of numerous applications, including cellular mRNA and miRNA quantification, biomarker discovery and validation, microbial quantification, cancer risk assessment, gene dosage determination, and detection of extremely low copy targets for forensic investigations.This, in turn, has resulted in an abundance of publications utilizing qPCR data obtained with diverse reagents, protocols, analysis methods, and reporting formats. Unfortunately, few papers report in detail how these results were obtained. This lack of clarity and transparency has led to concern in the research community over the reliability of qPCR data interpretation and the real danger of the scientific literature being corrupted with publications reporting erroneous and conflicting results.This has already occurred in some cases, resulting, for example, in retraction of a Science “Breakthrough of the Year 2005” report. Now that qPCR has come of age, standardization is needed to ensure its validity, prompting the recent formulation of guidelines to increase experimental transparency, promote consistency between laboratories, and therefore, help assure the publication of valid conclusions.

http://www.genengnews.com/articles/chitem.aspx?aid=2992
http://www.gene-quantification.de/miqe.html#press

MIQE - qPCR Assay Quality Assessment

The MIQE guidelines: minimum information for publication of quantitativereal-time PCR experiments.
Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT. (PDF)

Clin Chem. 2009 55(4): 611-622
http://www.rdml.org/miqe
http://miqe.gene-quantification.info
MIQE - media & press review

BACKGROUND: Currently, a lack of consensus exists on how best to perform andinterpret quantitative real-time PCR (qPCR) experiments. The problem isexacerbated by a lack of sufficient experimental detail in many publications,which impedes a reader's ability to evaluate critically the quality of theresults presented or to repeat the experiments.
CONTENT: The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelinestarget the reliability of results to help ensure the integrity of the scientific literature, promote consistency between laboratories, and increase experimentaltransparency. MIQE is a set of guidelines that describe the minimum informationnecessary for evaluating qPCR experiments. Included is a checklist to accompanythe initial submission of a manuscript to the publisher. By providing allrelevant experimental conditions and assay characteristics, reviewers can assess the validity of the protocols used. Full disclosure of all reagents, sequences,and analysis methods is necessary to enable other investigators to reproduceresults. MIQE details should be published either in abbreviated form or as anonline supplement.
SUMMARY: Following these guidelines will encourage betterexperimental practice, allowing more reliable and unequivocal interpretation of qPCR results.