qPCR / real-time PCR
Discussion board for any problem in quantitative real-time PCR. BLOG of www.Gene-Quantification.info - the reference in real time PCR, the academic and industrial information platform. The GQ pages describe and summarize all technical aspects involved in quantitative gene expression analysis using real-time qPCR, digital PCR & RT-qPCR.
Montag, 30. November 2020
Mittwoch, 12. August 2020
Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR
Relative Expression Software Tool (REST©) for group wise comparison
and statistical analysis of relative expression results in real-time PCR
Michael W. Pfaffl, Graham W. Horgan & Leo Dempfle (2002)
Nucleic Acids Research 2002 30(9): E36
Real-time reverse transcription followed by polymerase chain reaction (RT–PCR) is the most suitable method for the detection and quantification of mRNA. It offers high sensitivity, good reproducibility and a wide quantification range. Today, relative expression is increasingly used, where the expression of a target gene is standardised by a non-regulated reference gene. Several mathematical algorithms have been developed to compute an expression ratio, based on real-time PCR efficiency and the crossing point deviation of an unknown sample versus a control. But all published equations and available models for the calculation of relative expression ratio allow only for the determination of a single transcription difference between one control and one sample. Therefore a new software tool was established, named REST© (relative expression software tool), which compares two groups, with up to 16 data points in a sample and 16 in a control group, for reference and up to four target genes. The mathematical model used is based on the PCR efficiencies and the mean crossing point deviation between the sample and control group. Subsequently, the expression ratio results of the four investigated transcripts are tested for significance by a randomisation test. Herein, development and application of REST© is explained and the usefulness of relative expression in real-time PCR using REST© is discussed. The latest software version of REST© and examples for the correct use can be downloaded at http://relative.gene-quantification.info/
Montag, 10. August 2020
Virtual Symposium -- Diagnosing SARS-CoV-2 with qPCR
Diagnosing SARS-CoV-2 with qPCR
This virtual symposium, brought to you by The Scientist, will explore the limitations and challenges of current SARS-CoV-2 qPCR-based testing approaches, their root causes, and what can be learned for the future.
Tuesday, September 8, 2020
11:00 AM - 2:00 PM, Eastern Standard Time
https://www.the-scientist.com/sponsored-webinars/diagnosing-sars-cov-2-with-qpcr-symposium-67797
Stephen A. Bustin, PhD
Professor of Molecular Medicine
Faculty of Health, Education, Medicine and Social Care
Anglia Ruskin University
Jim F. Huggett, PhD
Senior Lecturer, Analytical Microbiology
School of Biosciences and Medicine
University of Surrey
Mikael Kubista, PhD
Head, Institute of Biotechnology
Czech Academy of Sciences
Founder and Chairman of the Board
TATAA Biocenter
Jo Vandesompele, PhD
Professor
Department of Biomolecular Medicine
Faculty of Medicine and Health Sciences
Ghent University
Michael W. Pfaffl, PhD
Professor of Molecular Physiology
Animal Physiology & Immunology
School of Life Sciences
Technical University of Munich
Mittwoch, 5. August 2020
Digital MIQE Guidelines Update -- Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020
Read our latest dMIQE2020 update in Clinical Chemistry
Digital MIQE Guidelines Update -- Minimum Information
for Publication of Quantitative Digital PCR Experiments for 2020
Freitag, 31. Juli 2020
MicroGenomics 2021 -- September 29th till October 1st in Florence
Microgenomics 2021 will be hosting a workshop on September 29th, 2021 at the University of Florence, Italy. We look forward to seeing you there!
And REMEMBER, The symposium is postponed to September 30-October 1, 2021!
And REMEMBER, The symposium is postponed to September 30-October 1, 2021!
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