Dienstag, 30. September 2014

Bio-Rad’s Universal Real-Time PCR App – Everything You Need to Know about Real-Time PCR at Your Fingertips

Bio-Rad’s Universal Real-Time PCR App – Everything You Need to Know about Real-Time PCR at Your Fingertips

Quickly find everything you need to know about real-time PCR at your fingertips with our Universal Real-Time PCR app. Download today!  http://bit.ly/1ttIemF

Bio-Rad’s Universal Real-Time PCR Reagents App brings you in-depth product information for all of our one-step and two-step qPCR kits and reagents. These reagents utilize our proprietary blend of passive reference dyes that enables their use on all real-time PCR systems.
Features of the app include:
  • Individual and competitive performance data compared to other leading products
  • Interactive tutorials covering the qPCR basics on how reverse transcription works and why not all cDNA synthesis kits are created equal
  • Technical manuals
  • Product inserts
  • Troubleshooting guides
  • And more ... ... 

Donnerstag, 18. September 2014

Optimization of Extraction of Circulating RNAs from Plasma - Enabling Small RNA Sequencing.


Optimization of Extraction of Circulating RNAs from Plasma - Enabling Small RNA Sequencing.
Spornraft M, Kirchner B, Haase B, Benes V, Pfaffl MW, Riedmaier I
PLoS One. 2014 9(9): e107259 - eCollection 2014

There are several protocols and kits for the extraction of circulating RNAs from plasma with a following quantification of specific genes via RT-qPCR. Due to the marginal amount of cell-free RNA in plasma samples, the total RNA yield is insufficient to perform Next-Generation Sequencing (NGS), the state-of-the-art technology in massive parallel sequencing that enables a comprehensive characterization of the whole transcriptome. Screening the transcriptome for biomarker signatures accelerates progress in biomarker profiling for molecular diagnostics, early disease detection or food safety. Therefore, the aim was to optimize a method that enables the extraction of sufficient amounts of total RNA from bovine plasma to generate good-quality small RNA Sequencing (small RNA-Seq) data. An increased volume of plasma (9 ml) was processed using the Qiagen miRNeasy Serum/Plasma Kit in combination with the QIAvac24 Plus system, a vacuum manifold that enables handling of high volumes during RNA isolation. 35 ng of total RNA were passed on to cDNA library preparation followed by small RNA high-throughput sequencing analysis on the Illumina HiSeq2000 platform. Raw sequencing reads were processed by a data analysis pipeline using different free software solutions. Seq-data was trimmed, quality checked, gradually selected for miRNAs/piRNAs and aligned to small RNA reference annotation indexes. Mapping to human reference indexes resulted in 4.8±2.8% of mature miRNAs and 1.4±0.8% of piRNAs and of 5.0±2.9% of mature miRNAs for bos taurus.

Mittwoch, 17. September 2014

qPCR NEWS -- September 2014 -- Hot papers in the field of microRNA and non-coding RNAs


The September 2014 newsletter informs about the latest news in quantitative real-time PCR (qPCR and RT-qPCR), which are compiled and summarised on the Gene Quantification domain. The focus of this newsletter issue is:
If this newsletter is not displayed correctly please use following LINK => qPCRnews.gene-quantification.info

Donnerstag, 24. Juli 2014

"BDQ Young Scientist Poster Awards" at qPCR & NGS 2015


"BDQ Young Scientist Poster Awards" at qPCR & NGS 2015
BDQ-awards.qPCR-NGS-2015.net

The new open access journal Biomolecular Detection and Quantification (BDQ) is proud to sponsor three BDQ Young Scientist Poster Awards at the qPCR & NGS 2015 Event.

The posters will be evaluated by an international jury on the basis of originality of the approach; quality of the work (e.g. appropriate methodology, interpretation of results, conclusiveness); quality of the presentation (e.g. clarity, response to questions); and self-reliance and independence (not one of co-authors, but the most active, if more than one author).

Eligible for the BDQ Young Scientist Poster Award:
The contest is open to students and early career scientists.

Award Presentation
BDQ Editor-in-Chief Stephen Bustin will award the three poster prizes on Wednesday afternoon directly after the lunch break March 25, 2015.

  1. BDQ Young Scientist Poster Award - First Prize
    The winner is awarded 700 Euros and a free submission to BDQ
  2. BDQ Young Scientist Poster Award – Second Prize
    The winner is awarded 200 Euros and a free submission to BDQ
  3. BDQ Young Scientist Poster Award – Third Prize
    The winner is awarded 100 Euros and a free submission to BDQ
Procedure for Application
Authors will be able to express their interest in participating in the BDQ Young Scientist Poster Award contest when submitting their abstract. Note that authors are only able to participate if their paper has been selected as a poster by the conference organizer.
Please register and submit your abstract using the Internet based ConfTool registration and submission platform => registration.qPCR-NGS-2015.net