Comparison of the miRNome and piRNome of bovine blood and plasma by small RNA sequencing.

Comparison of the miRNome and piRNome of bovine blood and plasma by small RNA sequencing.

Spornraft M, Kirchner B, Pfaffl MW, Riedmaier I.

Biotechnol Lett. 2015 Feb 21. [Epub ahead of print]

http://link.springer.com/article/10.1007/s10529-015-1788-2

OBJECTIVES:

The small RNAs of bovine plasma and whole blood were analysed using next-generation sequencing to quantify, profile and compare the microRNAs (miRNA) and piRNA signatures in both bio fluids.

RESULTS:

Evaluating read-count data resulted in a proportion of 5.0 ± 2.9 % of miRNAs in plasma while 38.2 ± 3.4 % were identified in whole blood. Regarding piRNAs, the percentages in both matrices were nearly the same: 1.4 ± 0.8 % of piRNAs in plasma and 1.9 ± 0.8 % in whole blood. Investigation of the ten most abundant miRNAs and piRNAs in both bio fluids revealed that two miRNAs and seven piRNAs were identical. Comparing the read-count values of these matching pairs highlighted that miRNA and piRNA levels in blood exceeded the abundance of their corresponding miRNAs and piRNAs in plasma, except liver-specific miR-122 and three piRNAs.

CONCLUSIONS:

The data strengthened evidence that the circulating small RNA signature in plasma is not only influenced by hematocytes and certain small RNAs could originate from other sources than cellular blood components.

Keynote Lectures -- Browse the qPCR & NGS 2015 Conference Agenda

qPCR & NGS 2015 Symposium
"Advanced Molecular Diagnostics for Biomarker Discovery"
7th international qPCR & NGS Event -- Symposium & Industrial Exhibition & Application Workshops 
23-27 March 2015
TUM School of Life Sciences, Technical University of Munich, Germany

Browse the Conference Agenda -- http://AgendaHTML.qPCR-NGS-2015.net
Print agenda PDF version  http://AgendaPDF.qPCR-NGS-2015.net

Keynote Lectures:

1. PCR in less than 30 seconds: Efficient, specific amplification with increased primer and polymerase.
   Carl T Wittwer; University of Utah, United States of America
2. Digital PLA  (Proximity Ligation Assay)
   Stephen Bustin; Anglia Ruskin University, Chelmsford, Essex, United Kingdom
3. Next-Generation RNA-Seq.
   Gary Schroth; Illumina, United States of America
4. How could digital PCR benefit clinical analysis?
   Jim Francis Huggett; LGC, United Kingdom
5. Updated evaluation of quantitative miRNA expression platforms in the microRNA quality control (miRQC) study.
   Jo Vandesompele; Ghent University on behalf of the microRNA quality control study consortium, Belgium
6. Mass Spectrometry Based Draft Of The Human Proteome.
   Bernhard Kuster; TU München, Freising Weihenstephan, Germany 
7. Next Generation Sequencing Applications to the study of human and animal Microbiomes.
   Karen E. Nelson, J. Craig Venter Institute, United States of America 
8. Expression Profiling of Circulating Tumor Cells: a Prognostic and Predictive Biomarker in Cancer.
   Mikael Kubista; TATAA Biocenter, Sweden
9. Variability of the Reverse Transcription Step: Practical Implications.
   Tania Nolan; The Gene Team, United Kingdom
10. Decoding lncRNA functions using high-throughput pathway perturbation.
    Pieter Mestdagh; Biogazelle, Belgium

-----------------------------------

Event Promotion Trailer  http://youtu.be/44nJ43gzXJQ
Download the event flyer  http://event-announcement.qPCR-NGS-2015.net
Event location on Google maps  http://goo.gl/maps/3p3gx
Register for Symposium & Workshops  http://registration.qPCR-NGS-2015.net

Class I odorant receptors, TAS1R and TAS2R taste receptors, are markers for subpopulations of circulating leukocytes.

Class I odorant receptors, TAS1R and TAS2R taste receptors, are markers for subpopulations of circulating leukocytes.

Malki A1, Fiedler J1, Fricke K1, Ballweg I1, Pfaffl MW1, Krautwurst D2

J Leukoc Biol. 2015 Jan 26. pii: jlb.2A0714-331RR. [Epub ahead of print]

http://www.ncbi.nlm.nih.gov/pubmed/25624459

http://www.jleukbio.org/content/early/2015/01/24/jlb.2A0714-331RR.long

Our cellular immune system has to cope constantly with foodborne substances that enter the bloodstream postprandially. Here, they may activate leukocytes via specific but yet mostly unknown receptors. Ectopic RNA expression out of gene families of chemosensory receptors, i.e., the ∼400 ORs, ∼25 TAS2R bitter-taste receptors, and the TAS1R umami- and sweet-taste receptor dimers by which we typically detect foodborne substances, has been reported in a variety of peripheral tissues unrelated to olfaction or taste. In the present study, we have now discovered, by gene-specific RT-PCR experiments, the mRNA expression of most of the Class I ORs (TAS1R) and TAS2R in 5 different types of blood leukocytes. Surprisingly, we did not detect Class II OR mRNA. By RT-qPCR, we show the mRNA expression of human chemosensory receptors and their cow orthologs in PMN, thus suggesting an evolutionary concept. By immunocytochemistry, we demonstrate that some olfactory and taste receptors are expressed, on average, in 40-60% of PMN and T or B cells and largely coexpress in the same subpopulation of PMN. The mRNA expression and the size of subpopulations expressing certain chemosensory receptors varied largely among individual blood samples, suggesting a regulated expression of olfactory and taste receptors in these cells. Moreover, we show mRNA expression of their downstream signaling molecules and demonstrate that PTX abolishes saccharin- or 2-PEA-induced PMN chemotactic migration, indicating a role for Gi-type proteins. In summary, our data suggest "chemosensory"-type subpopulations of circulating leukocytes.

January 2015 newsletter -- Exosomes in Molecular Diagnostics & Biomarker Discovery

Dear researcher,

The January 2015 newsletter informs about the latest news in quantitative real-time PCR (qPCR and RT-qPCR), which are compiled and summarised on the www.Gene-Quantification.info
The focus of this newsletter issue is:

  * NEW -- Exosomes in Molecular Diagnostics & Biomarker Discovery --  http://Exosomes.Gene-Quantification.info
  * Abstract Call -- qPCR & NGS Symposium in March 2015 -- http://Call.qPCR-NGS-2015.net
  * "Biomolecular Detection and Quantification" -- Call for submissions - http://BDQ.Gene-Quantification.info
  * GenEx 6  -- The most powerful tool for complex qPCR data analysis - download a free trial version - http://GenEx.Gene-Quantification.info

Streaming portal
Join the new eConferences streaming webportal -- http://www.eConferences.de -- Amplify your knowledge!
This portal is dedicated to scientists from the community of qPCR, digital-PCR, NGS and Molecular Diagnostics. You’ll find here all the records from around 200 presentations held at qPCR & NGS Events in the past years. We provide the presentations via movie streaming technology in high quality - high resolution and perfect sound quality in high speed - on any internet browser or mobile device, including Android or iOS.

... read the full qPCR newsletter -- http://qPCRnews.gene-quantification.info

Register for the qPCR & NGS 2015 Event
... ... and submit your talk or poster abstract!

• CALL.qPCR-NGS-2015.net
• Abstract submission deadline -- 31st Jan 2015
• Early & discount registration period will end -- 31st Jan 2015
• YouTube Promotion Trailer -- qPCR & NGS 2015

---

qPCR & NGS 2015 Event
"Advanced Molecular Diagnostics for Biomarker Discovery"
7th international qPCR & NGS Event -- Symposium & Industrial Exhibition & Application Workshops
23-27 March 2015
TUM School of Life Sciences Weihenstephan
Technical University of Munich, Freising, Germany
www.qPCR-NGS-2015.net

Talk and Poster will be presented in the following scientific sessions:

• Main topic:   Advanced Molecular Diagnostics
• Main topic:   Biomarker Discovery
• Main topic:   Next Generation Sequencing (NGS)
   - NGS overview talks - information technology in the era of NGS
   - Pre NGS - sample prep & setup & library generation
   - NGS - new sequencing technologies (e.g. single molecule and pore sequencing)
   - NGS - data analysis (data management, mapping, alignment algorithms, data de novo assembly )
   - NGS - diagnostic applications
• MicroGenomics & Single-cells diagnostics
• Circulating Nucleic Acids
• Molecular Diagnostics in Agriculture, Veterinary Medicine, Food & Environmental Science
• MIQE & QM & Standardisation strategies in molecular diagnostics
• Digital PCR  &  Nano-fluidics
• Non-coding RNAs -- microRNA, small RNAs, long non-coding RNAs
• qPCR Data Analysis -- BioStatistics & BioInformatics

Please register using the Internet based ConfTool registration & submission platform => Registration.qPCR-NGS-2015.net