qPCR / real-time PCR

Dienstag, 10. Dezember 2019

3rd International Symposium on Microgenomics, September 10-11, 2020 @ Florence, Italy

Microgenomics 2020
Tissue, cell and circulating biomarkers -- the challenge of single cell and small sample analysis.
3rd International Symposium on Microgenomics, September 10-11, 2020 @ Florence, Italy
and various Workshops on September 9th
https://www.MicroGenomics2020.com

We are pleased to announce the 3rd International Symposium on Microgenomics on 10-11 September 2020, in Florence, Italy preceded by a technical workshop on September 9th.
Join us for this major European event in the field!

Montag, 12. August 2019

MIQE & qPCR iBook -- 3rd Edition 12. July 2019

Download our updated MIQE & qPCR iBook
How to apply the MIQE guidelines - a visual, interactive and practical qPCR guide
Editors: Afif M. Abdel Nour & Michael W. Pfaffl
3rd Edition (12. July 2019)
ISBN 9783000488061
Free download -- https://books.apple.com/de/book/miqe-qpcr/id993276375
An ePub version will follow soon!
#MIQE #qPCR #dPCR #ddPCR

Mittwoch, 22. Mai 2019

Download a free 14-day trial version of GenEx 7.0 -- The ultimate tool for qPCR data analysis!

GenEx – the ultimate tool for qPCR data analysis
Mikael Kubista and Amin Forootan
1 TATAA Biocenter AB, Göteborg, Sweden; 2 MultiD Analyses AB, Göteborg, Sweden

Download a free GenEx 7.0 -- 14-day trial version @ GenEx.gene-quantification.info

GenEx was the first commercial software developed for qPCR, and has since then become market leader with few thousands of users and several thousands of publications using GenEx. Sup- ported by all leading qPCR instrument manufacturers GenEx has batch import and corrects for inter-run variation. During pre- processing missing values are imputed, outliers are handled, and data are optimally normalized. Absolute quantification includes determining limit of detection, limit of quantification and dynamic range. All parameters are determined with confidence ranges in line with CLSI, CEN and ISO guidelines. Expression data are ana- lyzed with univariate methods including t-test, non-parametric methods and various ANOVAs with appropriate corrections for multiple testing ambiguity or multivariate methods including hierarchical clustering, Principal Component Analysis (PCA), Self- Organizing Map (SOM), Artificial Neural Network (ANN), Support Vector Machines (SVM), and many more. All analyses are easily per- formed with few clicks with the mouse using intuitive workflows that generate results that are readily interpreted in a biologi- cal context. At this lunch seminar, we will present GenEx and exemplify how easily multiple run files are in imported, measured data pre-processed, and how data are interactively analyzed using dynamic PCA to reveal key expression pathways and correlations across samples. All participants will receive FREE GenEx license for limited time.

The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. SA Bustin et al., Clinical Chemistry 55 (4), 611-622