Donnerstag, 16. Februar 2017

qPCR dPCR NGS 2017 Event Agenda is Online


8th Gene Quantification Event Agenda is Online #GQ2017
72 Talks and 67 Posters around "Liquid Biopsy, Integrative Big Data Analysis in qPCR and NGS, Biomarker Signature, single-cell qPCR, ... and beyond"
Agenda.qPCR-dPCR-NGS-2017.net

"Late Poster Submissions" will be accepted until 16th March 2017  
Submission.qPCR-dPCR-NGS-2017.net
These will NOT appear in the BDQ Supplement Issue

Montag, 23. Januar 2017

Final TALK & POSTER Abstract Call -- qPCR dPCR NGS 2017


deadline 31st Jan 2017


Scientific Symposium Sessions:
- Liquid Biopsy & Circulating Nucleic Acids
- Advanced Molecular Diagnostics
- Integrative Big Data Analysis
- Biomarker Signatures
- Digital PCR
- Non-coding RNAs -- microRNA, isomiRs, small RNAs, long non-coding RNAs
- MicroGenomics & Single-Cells Diagnostics
- Next Generation Sequencing (NGS)
- Molecular Diagnostics in Life Science
- MIQE & QM & Standardisation Strategies in Molecular Diagnostics

Register and submit your scientific contribution (talk or poster abstracts) http://registration.qPCR-dPCR-NGS-2017.net

Donnerstag, 15. Dezember 2016

MIQE & qPCR iBook -- What's New in Version 2.5


MIQE & qPCR
How to apply the MIQE guidelines - a visual, interactive and practical qPCR guide

2nd edition (version 2.5 published 12th Dec 2016)
Editors:  Afif M. Abdel Nour & Michael W. Pfaffl
ISBN 9783000488061
Free download via iTunes  https://itunes.apple.com/book/miqe-qpcr/id993276375?mt=11


What's New in Version 2.5

The present second edition of the MIQE & qPCR book should help to spread this MIQE idea even further in any laboratory worldwide and beyond in the scientists’ workflow and minds. It should clearly show how to apply the guidelines and serve as a handy, visual, interactive and practical guide. For now in the first year after publication of the first edition we could count more than 1200 downloads of the MIQE & qPCR book from more than 30 countries. Our goal for the second edition is to update the existing content by new chapters, and to improve this fancy interactive tool, interfacing scientific publications with educating pictures, videos and scientific talks. We implemented multiple new chapters, describing the significance of the reverse transcription reaction, why qPCR assay validation is so important for high sensitivity and good reproducibility, and one reviewing chapter about the necessity of performing quality control at all levels in the qPCR workflow.

Mittwoch, 14. Dezember 2016

qPCR dPCR NGS 2017 -- Call for POSTER & TALK Abstracts




Scientific Symposium Sessions:
- Liquid Biopsy & Circulating Nucleic Acids
- Advanced Molecular Diagnostics
- Integrative Big Data Analysis
- Biomarker Signatures
- Digital PCR
- Non-coding RNAs -- microRNA, isomiRs, small RNAs, long non-coding RNAs
- MicroGenomics & Single-Cells Diagnostics
- Next Generation Sequencing (NGS)
- Molecular Diagnostics in Life Science
- MIQE & QM & Standardisation Strategies in Molecular Diagnostics

Register and submit your scientific contribution (talk or poster abstracts) => http://registration.qPCR-dPCR-NGS-2017.net

Valid biomarker signatures from liquid biopsies - how to standardize next generation sequencing


Dominik Buschmann1,2, Benedikt Kirchner1,3, Michael W. Pfaffl1
1 Department of Animal Physiology and Immunology, TUM School of Life Sciences Weihenstephan, Freising, Germany
2 Institute of Human Genetics, University Hospital, Ludwig-Maximilians-University Munich, Munich, Germany
3 Dr. von Hauner Children's Hospital, Ludwig-Maximilians-University Munich, Munich, Germany

The advent of Next-Generation Sequencing (NGS) techniques has revolutionized transcriptomics research and opened numerous avenues for scientific and clinical applications. While reverse transcriptase quantitative real-time PCR (RT-qPCR) is still considered the gold standard of gene expression analysis, its high throughput, single-nucleotide resolution and ever-plummeting costs have made NGS an intriguing and increasingly accessible alternative to this classical method. In addition to mere transcript quantification, RNA-Seq offers exciting new insights such as the discovery of novel transcripts and detection of alternative splice variants or chimeric transcripts. While DNA sequencing yields fascinating discoveries about the genomic makeup of target tissues, RNA-Seq might hold even more potential for biomarker research and drug discovery. 

... more about Liquid Biopsies Liquid-Biopsy.Gene-Quantification.info