We recently had a chance to interview Jim Hugget, Ph.D., Science Leader, Nucleic Acid Metrology, LGC to get his opinion on how digital PCR can increase standardization in biological metrology.
Freitag, 7. März 2014
Donnerstag, 6. März 2014
new open access journal -- Biomolecular Detection and Quantification
Biomolecular Detection and Quantification
The new online, open access, peer-reviewed journal in molecular methodology applications
Editors: Stephen Bustin, Jim Huggett, Justin O'Grady, Michael Pfaffl, Carl Witwer, Ron Cook
http://www.journals.elsevier.com/biomolecular-detection-and-quantification/
Biomolecular Detection and Quantification (BDQ) is an open access, peer-reviewed international journal dedicated to championing excellence in molecular study design, measurement, data analysis and reporting. Its focus is on the application of qualitative and quantitative molecular methodologies to all areas of clinical and life sciences. The journal has two main aims:
The new online, open access, peer-reviewed journal in molecular methodology applications
Editors: Stephen Bustin, Jim Huggett, Justin O'Grady, Michael Pfaffl, Carl Witwer, Ron Cook
http://www.journals.elsevier.com/biomolecular-detection-and-quantification/
Biomolecular Detection and Quantification (BDQ) is an open access, peer-reviewed international journal dedicated to championing excellence in molecular study design, measurement, data analysis and reporting. Its focus is on the application of qualitative and quantitative molecular methodologies to all areas of clinical and life sciences. The journal has two main aims:
- to provide a forum for discussion and recommendation of guidelines designed to improve the accuracy of molecular measurement, its data analysis and the transparency of its subsequent reporting;
- to publish molecular biology based studies that adhere to best practice guidelines, both current and future.
Freitag, 28. Februar 2014
EMBO Workshop on Simultaneous profiling of RNA and protein using proximity ligation assay
EMBO Workshop on Simultaneous profiling of RNA and protein using proximity ligation assay
7 July 2014 | Chelmsford, United Kingdom
Registration is now open for this EMBO Workshop, please check out the website here:
http://events.embo.org/14-pla
This EMBO workshop will cover the background to PLA, introduce the concept of quantitative protein detection and aims to inaugurate MIQE-style guidelines for PLA. The presenters will be scientists who have extensive experience of the technique. Examples of studies will be presented to illustrate the use of PLA for simultaneous detection of RNA and protein in diverse sample types, including single cells, focusing on technical requirements for a functioning assay.
7 July 2014 | Chelmsford, United Kingdom
Registration is now open for this EMBO Workshop, please check out the website here:
http://events.embo.org/14-pla
This EMBO workshop will cover the background to PLA, introduce the concept of quantitative protein detection and aims to inaugurate MIQE-style guidelines for PLA. The presenters will be scientists who have extensive experience of the technique. Examples of studies will be presented to illustrate the use of PLA for simultaneous detection of RNA and protein in diverse sample types, including single cells, focusing on technical requirements for a functioning assay.
Donnerstag, 27. Februar 2014
Careful strategizing helps make PCR sensitive enough for the most challenging experiments
PCR: paths to sensitivity
by Vivien Marx
TECHNOLOGY FEATURE Nature Methods 11, 241–245 (2014) doi:10.1038/nmeth.2849
Published online 27 February 2014
Careful strategizing helps make PCR sensitive enough for the most challenging experiments.
Scientists may think PCR is simple, says Ghent University researcher Jo Vandesompele, “but it's not.” He splits his time between his own research and Biogazelle, a university spin-out that he cofounded and of which he is CEO. His team developed qPCR analysis software called qbase+, and he has a statistical algorithm and software for dPCR in the works. He consults and sponsors training in PCR techniques to help a lab or a company department standardize its practices and to strategize about experiments. Courses have focused on qPCR best practices, and this year he is adding dPCR.
http://www.nature.com/nmeth/journal/v11/n3/full/nmeth.2849.html
by Vivien Marx
TECHNOLOGY FEATURE Nature Methods 11, 241–245 (2014) doi:10.1038/nmeth.2849
Published online 27 February 2014
Careful strategizing helps make PCR sensitive enough for the most challenging experiments.
Scientists may think PCR is simple, says Ghent University researcher Jo Vandesompele, “but it's not.” He splits his time between his own research and Biogazelle, a university spin-out that he cofounded and of which he is CEO. His team developed qPCR analysis software called qbase+, and he has a statistical algorithm and software for dPCR in the works. He consults and sponsors training in PCR techniques to help a lab or a company department standardize its practices and to strategize about experiments. Courses have focused on qPCR best practices, and this year he is adding dPCR.
http://www.nature.com/nmeth/journal/v11/n3/full/nmeth.2849.html
Freitag, 21. Februar 2014
Do TaqMan® Assays Comply with MIQE Guidelines?
After introducing MIQE guidelines, a set of standards for producing and publishing qPCR data, Sr. Field Applications Specialist Doug Rains explains how Life Technologies' large and diverse collection of TaqMan® assays comply with these guidelines. Learn about Life Technologies' TaqMan assays guarantee program and the rigorous bioinformatics process through which each TaqMan® assays is designed.
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