Montag, 22. Juni 2015

RDML-Ninja and RDMLdb for standardized exchange of qPCR data.

RDML-Ninja and RDMLdb for standardized exchange of qPCR data.
Ruijter JM, Lefever S, Anckaert J, Hellemans J, Pfaffl MW, Benes V, Bustin SA, Vandesompele J, Untergasser A; and RDML consortium.
BMC Bioinformatics. 2015 16: 197

BACKGROUND: The universal qPCR data exchange file format RDML is today well accepted by the scientific community, part of the MIQE guidelines and implemented in many qPCR instruments. With the increased use of RDML new challenges emerge. The flexibility of the RDML format resulted in some implementations that did not meet the expectations of the consortium in the level of support or the use of elements.

RESULTS: In the current RDML version 1.2 the description of the elements was sharpened. The open source editor RDML-Ninja was released ( ). RDML-Ninja allows to visualize, edit and validate RDML files and thus clarifies the use of RDML elements. Furthermore RDML-Ninja serves as reference implementation for RDML and enables migration between RDML versions independent of the instrument software. The database RDMLdb will serve as an online repository for RDML files and facilitate the exchange of RDML data ( ). Authors can upload their RDML files and reference them in publications by the unique identifier provided by RDMLdb. The MIQE guidelines propose a rich set of information required to document each qPCR run. RDML provides the vehicle to store and maintain this information and current development aims at further integration of MIQE requirements into the RDML format.

CONCLUSIONS: The editor RDML-Ninja and the database RDMLdb enable scientists to evaluate and exchange qPCR data in the instrument-independent RDML format. We are confident that this infrastructure will build the foundation for standardized qPCR data exchange among scientists, research groups, and during publication.

Dienstag, 12. Mai 2015

MIQE & qPCR iBook (1st edition)

MIQE & qPCR iBook

How to apply the MIQE guidelines - a visual, interactive and practical qPCR guide

1st edition by Afif M. Abdel Nour & Michael W. Pfaffl
ISBN 9783000488061
Free download on iTunes

Throughout the past 30 years Polymerase Chain Reaction (PCR) has proven to be the most powerful technique in a scientist toolbox. Only few techniques had a comparable success story like PCR. This iBook will guide you in better practicing in your laboratory thanks to the MIQE guideline.

More qPCR books and iBooks

Montag, 11. Mai 2015

Advances in Oncology & Symposium on Molecular Medicine

20th World Congress on Advances in Oncology &
18th International Symposium on Molecular Medicine
October 8-10, 2015
Metropolitan Hotel, Athens, Greece

Pre-Congress qPCVR Workshops
in cooperation with the TATAA Biocenter, Sweden
6-7 October 2015
‘MIQE - How to get good-quality control in qPCR’ (2-day workshop by Dr. Kristina Lind)
‘Discovery and Validation of Expression Biomarkers — Experimental Design and Data Analysis’ (2-day workshop by Prof. Mikael Kubista)

Industrial Exhibition
In parallel to the scientific symposium of 2015, an Industrial Exhibition will take place, at which international companies, leaders in the fields of Oncology and Molecular Medicine, will present their latest technologies and services in clinical diagnostics. If you are interested in joining the exhibition

Contact Details

Metropolitan Hotel, Athens, Greece

Dienstag, 31. März 2015

First impressions from qPCR & NGS 2015 Event

First impressions from qPCR & NGS 2015 Event
23rd - 27th March 2015
480 participants from 38 countries

Talk Agenda --
Poster Session --

Montag, 16. März 2015

qPCR, dPCR, NGS – A journey

qPCR, dPCR, NGS – A journey
Jim F. Huggett, Justin O’Grady, Stephen Bustin
Biomolecular Detection and Quantification, available online 15 January 2015

Scientific conferences fulfill many roles, but one of the most important ones is that they help shape the direction in which a scientific discipline grows by promoting person-to-person exchanges of information, ideas and constructive criticisms between scientists from different backgrounds. This interaction also helps to identify areas of controversy and promotes efforts to address and, it is hoped, resolve them. This year is the 30th anniversary of the publication of the first practical description of the polymerase chain reaction [1], arguably one of the simplest and the most widely used molecular technology. It also sees the 7th instalment of the Freising PCR meetings, which are the longest established, continuous and most influential conferences in this field and have provided a looking glass for conceptual and technical innovation as well as practical applications of PCR-associated methods.