Dienstag, 16. Dezember 2014

qPCR NEWS -- Dec 2014 -- Hot papers in qPCR data analysis and interpretation tools


Our newsletter informs about the latest news in quantitative real-time PCR (qPCR and RT-qPCR), which are compiled and summarised on the www.Gene-Quantification.info
The focus of the December 2014 qPCR newsletter issue is:

Join the new eConferences streaming webportal -- www.eConferences.de -- Amplify your knowledge!

This portal is dedicated to scientists from the community of qPCR, digital-PCR, NGS and Molecular Diagnostics. You’ll find here all the records from around 200 presentations held at qPCR & NGS Events in the past years.
We provide the presentations via movie streaming technology in high quality - high resolution and perfect sound quality in high speed - on any internet browser or mobile device, including Android or iOS.


If this newsletter is not displayed correctly by your email client, please use following qPCRnews.gene-quantification.info

Montag, 8. Dezember 2014

The Scientist’s annual Top 10 Innovations competition

http://www.the-scientist.com/?articles.view/articleNo/41486/title/Top-10-Innovations-2014/

The Scientist’s annual Top 10 Innovations competition has again turned up some exciting new products that are poised to revolutionize the work of life scientists. Familiar names, such as Illumina and Leica, win again with updates to their sequencing and imaging technologies, while newcomers like Sciencescape, Organovo, and Edico Genome debut with novel products that caught the eyes of our independent, expert judges.
The Top 5 of this year’s winning innovations involve the process of genome sequencing: tools to do the actual sequencing, technologies to make it easier to prepare genetic regions for sequencing, and a processor that can handle the avalanche of data that results from such analyses. The Dragen Bio-IT Processor can cut genome analysis to a fraction of the time it takes using older technologies—a feat that earns it top billing in this year’s contest. San Diego–based Illumina garners the second and third spots with its latest sequencers, one of which (the third-place HiSeq X 10) achieves the long-awaited $1,000 human genome milestone.
Other cool tech rounds out the 2014 Top 10 innovations, from a human liver model that remains phenotypically stable for 40 days, to a literature-management tool that makes keeping track of the flood of papers coming out in any field as easy as following a Twitter feed.
It was a tough job for our panel of expert judges to pick the 10 best life-science products in a year when so many innovations came down the pike, but as ever, the cream has risen to the top. Read all about the winners of the Top 10 Innovations for 2014, and be sure to visit www.the-scientist.com for lots of extra Top 10 information, including expanded comments from our judges and a look at products that earned honorable mentions in this year’s contest.
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Samstag, 22. November 2014

qPCR NEWS Nov 1014 -- Hot papers around MIQE and qPCR QC



qPCR NEWS Nov 2014 -- focus on hot papers around MIQE and qPCR QC

Dear researcher,
dear Gene Quantification page reader,

Our newsletter informs about the latest news in quantitative real-time PCR (qPCR and RT-qPCR), which are compiled and summarised on the www.Gene-Quantification.info
The focus of this newsletter issue is:

* MIQE and further hot papers in the field of QC -- http://MIQE.gene-quantification.info
* MIQE press release in 2014 -- http://MIQE-press.gene-quantification.info
* A nice review about qPCR data analysis tools -- http://Bioinformatics.gene-quantification.info
* Join our next event -- qPCR & NGS 2015 -- "Advanced Molecular Diagnostics for Biomarker Discovery" - http://www.qPCR-NGS-2015.net
* "Biomolecular Detection and Quantification" -- Call for submissions - http://BDQ.Gene-Quantification.info
* GenEx 6  -- The most powerful tool for complex qPCR data analysis - download a free trial version - http://GenEx.Gene-Quantification.info


Join the new eConferences streaming webportal -- http://www.eConferences.de -- Amplify your knowledge!
This portal is dedicated to scientists from the community of qPCR, digital-PCR, NGS and Molecular Diagnostics. You’ll find here all the records from around 200 presentations held at qPCR & NGS Events in the past years. We provide the presentations via movie streaming technology in high quality - high resolution and perfect sound quality in high speed - on any internet browser or mobile device, including Android or iOS.


If this newsletter is not displayed correctly by your email client, please use following http://qPCRnews.gene-quantification.info

Montag, 17. November 2014

Study Shows High Variability in Reverse Transcription Efficiency

Study Shows High Variability in Reverse Transcription Efficiency
Nov 13, 2014 via GenomeWeb
http://www.clinchem.org/content/early/2014/10/28/clinchem.2014.230615.abstract

Though reverse transcription of RNA to cDNA is an essential first step for a growing number of genomics applications, researchers have long known of problems associated with RT and its effects on study results. Now, a new study has examined some of those issues and has provided a workflow and guidelines for researchers publishing RT-based data.

The study, published in Clinical Chemistry late last month, was undertaken by some of the authors of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments, or MIQE, guidelines, which debuted in 2009. It compared measures of different mRNA targets using six commercially available RT enzymes and varying sample concentrations of differing qualities. Results showed the variability was "sufficiently large to call into question the validity of many published data that rely on quantification of cDNA."
... ... read more
https://www.genomeweb.com/pcrsample-prep/study-shows-high-variability-reverse-transcription-efficiency