Donnerstag, 28. Juli 2016

How to apply the MIQE guidelines - a visual, interactive and practical qPCR guide

2nd edition published 26th July 2016
Editors:  Afif M. Abdel Nour & Michael W. Pfaffl
ISBN 9783000488061
Free download via iTunes

more info here

MIQE & qPCR Preface:   The qPCR and dPCR MIQE guidelines – A success story!
(Editorial for 2nd edition published in July 2016)

The MIQE guidelines and the resulting scientific validity will be supported by more and more researchers, biological journals, academic and commercial institutions. Today in July 2016 we count more than 4600 citations for the MIQE guideline applied in qPCR and around160 citations for the digital PCR (dPCR) MIQE guideline (measured by Google Scholar). Hence the qPCR and dPCR MIQE guidelines are a worldwide full success story which will be driven forward by the scientific community, e.g.

The present second edition of the MIQE & qPCR iBook should help to spread this MIQE idea even further in any laboratory worldwide and beyond in the scientists’ workflow and minds. It should clearly show how to apply the guidelines and serve as a handy, visual, interactive and practical guide. For now in the first year after publication of the first edition we could count more than 1200 downloads of the MIQE & qPCR iBook from more than 30 countries. Our goal for the second edition is to update the existing content by new chapters, and to improve this fancy interactive tool, interfacing scientific publications with educating pictures, videos and scientific talks. We implemented multiple new chapters, describing the significance of the reverse transcription reaction, why PCR assay validation is so important for high sensitivity and good reproducibility, and one reviewing chapter about the necessity of performing quality control at all levels in the qPCR workflow. In summary we are proud to present a selection of international highly recognized authors from the academic field as well from industrial research presenting their latest applications. Described qPCR / dPCR methods and applications are linked to the MIQE context and show it on the basis of educational questionnaires or interactive ‘how to do’ instruction sheets. The at-hand MIQE & qPCR iBook should deliver the MIQE guidelines directly to the researcher and help to solve the daily problems in the molecular biology laboratory using quantitative PCR, digital PCR, single-cell qPCR, microRNA applications or any comparable techniques using PCR.

We hope you like our explanatory, interactive and educational iBook concept, showing the advantages of the MIQE guidelines in an easy and understandable way, and to guarantee the successful qPCR or dPCR application at the lab bench.

The editors
Afif M. Abdel Nour & Michael W. Pfaffl

Mittwoch, 29. Juni 2016

qPCR Newsletter June 2016 -- Focus on Liquid Biopsy

Dear researcher,

Our newsletter informs about the latest news in gene expression profiling using qPCR and related methods, which are compiled and summarised on the 
Gene Quantification domain. The focus of this qPCR NEWS issue is:

 NEW section -- What can we learn from liquid biopsies? --
 Join our next Gene Quantification Event in April 2017 --
 Call for Papers -- Biomolecular Detection and Quantification --
 GenEx 6.1 -- The most powerful tool for complex qPCR data analysis  => download a free trial version -- 


Liquid biopsy is a minimally invasive technology for detection of molecular biomarkers without the need for costly or invasive procedures. It is a simple and non-invasive alternative to surgical biopsies which enables medical doctors to discover a range of information about a disease or a tumour through a simple blood sample. Circulating cancer cells or traces of the cancer’s RNA or DNA in the blood can give clues about which treatments are most likely to work for that patient. Circulating nucleic acids are protected by extracellular micro-vesicles, mainly exosomes.

New dedicated methods enable you to enrich and purify from this liquid biopsy:
• circulating free DNA (cfDNA)
• circulating small-RNA
• circulating tumor cells (CTCs)
• extracellular mirco-vesicles (including exosomes) containing small-RNA, mRNA and DNA

What can we learn from liquid biopsies?
by Marc Beishon, CancerWorld
Early detection, disease prognosis, a guide to treatment, a key to unlock the secrets of how cancers evolve. Researchers have high hopes for what they can learn from the biological detritus shed by primary tumours and metastases.  ... ... more info

Montag, 27. Juni 2016

qPCR dPCR & NGS 2017 -- 8th international Gene Quantification Event

qPCR dPCR & NGS 2017
8th international Gene Quantification Event
Symposium & Industrial Exhibition & Application Workshops
Main topics:   Liquid Biopsy, Integrative Big Data Analysis, Biomarker Signature … and beyond
3 - 7 April 2017, in Freising-Weihenstephan,
School of Life Sciences, Technical University of Munich, Weihenstephan, Germany

Scientific Symposium Sessions:
- Liquid Biopsy & Circulating Nucleic Acids
- Advanced Molecular Diagnostics
- Integrative Big Data Analysis
- Biomarker Signatures
- Digital PCR
- Non-coding RNAs -- microRNA, isomiRs, small RNAs, long non-coding RNAs
- MicroGenomics & Single-Cells Diagnostics
- Next Generation Sequencing (NGS)
- Molecular Diagnostics in Life Science
- MIQE & QM & Standardisation Strategies in Molecular Diagnostics

Register and submit your scientific contribution (talk or poster abstracts) =>

qPCR dPCR & NGS 2017 Event is the world most recognized Gene Quantification Event with an average audience of more than 500 participants from all over the world. The content covers all types of genomics applications from quantitative RT-PCR, over digital PCR to the latest Next Generation Sequencing technologies and the connected integrative Big Data Analysis. As done in previous years, we offer a three day scientific symposium with around 70 talks in two lecture halls. Parallel to the scientific symposium an Industrial Exhibition will take place where around 35-40 international companies will present their newest qPCR, dPCR and NGS services, hardware and technologies. The symposium will be followed by various 2-day PCR & NGS related Workshops taking place April 6th and 7th powered by leaders in the field

Event location is the central lecture hall complex at TUM School of Life Sciences Technical University of Munich in Freising Weihenstephan, Germany The TUM and the Biotech region around Munich are part of the largest Biotech cluster in Europe BioM representing more than 250 companies and academic institutions, located close to the Munich airport (MUC) directly in the heart of Bavaria.

If you have further questions, we are pleased to help you. Up to date information about the EXHIBITION & SPONSORING, is available on the Symposium Homepage or contact us via

Samstag, 18. Juni 2016

Toward reliable biomarker signatures in the age of liquid biopsies

Toward reliable biomarker signatures in the age of liquid biopsies - how to standardize the small RNA-Seq workflow
Dominik Buschmann, Anna Haberberger, Benedikt Kirchner, Melanie Spornraft, Irmgard Riedmaier, Gustav Schelling and Michael W. Pfaffl
Nucleic Acids Research, June 17, 2016 doi:10.1093/nar/gkw545

Small RNA-Seq has emerged as a powerful tool in transcriptomics, gene expression profiling and biomarker discovery. Sequencing cell-free nucleic acids, particularly microRNA (miRNA), from liquid biopsies additionally provides exciting possibilities for molecular diagnostics, and might help establish disease-specific biomarker signatures. The complexity of the small RNA-Seq workflow, however, bears challenges and biases that researchers need to be aware of in order to generate high-quality data. Rigorous standardization and extensive validation are required to guarantee reliability, reproducibility and comparability of research findings. Hypotheses based on flawed experimental conditions can be inconsistent and even misleading. Comparable to the well-established MIQE guidelines for qPCR experiments, this work aims at establishing guidelines for experimental design and pre-analytical sample processing, standardization of library preparation and sequencing reactions, as well as facilitating data analysis. We highlight bottlenecks in small RNA-Seq experiments, point out the importance of stringent quality control and validation, and provide a primer for differential expression analysis and biomarker discovery. Following our recommendations will encourage better sequencing practice, increase experimental transparency and lead to more reproducible small RNA-Seq results. This will ultimately enhance the validity of biomarker signatures, and allow reliable and robust clinical predictions.

Montag, 25. April 2016

qPCR NEWS April 2016 -- Powerful tools -- MIQE press review -- BDQ in PMC

Dear researcher,

Our newsletter informs about the latest news in gene expression profiling using qPCR and related methods, which are compiled and summarised on
The focus of this qPCR NEWS issue is:
  • Powerful tools --
    • The inclusion of transcriptome anaylsis datasets to the Human Protein Atlas database makes it even more comprehensive.
    • MTD -- A mammalian transcriptomic database to explore gene expression and regulation.
  • MIQE Press Review - Find a summary of MIQE Press Review and related publications -
  • Now listed in PMC -- Biomolecular Detection and Quantification --      
  • Call for Papers -- Biomolecular Detection and Quantification --
    • Recent Advances in MicroGenomics "Molecular Analysis at the Tissue-specific and Single Cell Level"
  • GenEx 6.1 -- The most powerful tool for complex qPCR data analysis  => download a free trial version --
Join the updated eConferences streaming webportal -- Amplify your knowledge in  qPCR, dPCR and NGS!
This streaming portal is dedicated to scientists from the community of qPCR, digital PCR, Next Generation Sequencing (NGS), MicroGenomics (MG) and Molecular Diagnostics (MDx). You’ll find here all the records of 280 presentations held at qPCR & NGS and MG Events in the past years – qPCR 2010 in Vienna  to qPCR & NGS 2015 in Freising-Weihenstephan.
We provide the presentations via movie streaming technology in high quality – high resolution and perfect sound quality in high speed – on any internet browser or mobile device.