Mittwoch, 22. Juli 2015

Free available talks held at qPCR & NGS 2015 Event

qPCR & NGS 2015 Event
“Advanced Molecular Diagnostics for Biomarker Discovery”
7th international qPCR & NGS Event
Symposium & Industrial Exhibition & Application Workshops


Digital PCR
  http://www.econferences.de/digital-pcr-2015/
Optimisation, MIQE and Quality Control Strategies in qPCR
  http://www.econferences.de/optimisation-miqe-qc-strategies-in-qpcr-2015/
qPCR Biostatistics and BioInformatics
  http://www.econferences.de/qpcr-biostatistics-bioinformatics-2015/



Mittwoch, 15. Juli 2015

RNA Interference Off-target Screening using Principal Component Analysis | Open Access | OMICS Publishing Group

RNA Interference Off-target Screening using Principal Component Analysis
Jakob Müller* and Michael W. Pfaffl
Physiology Weihenstephan, Technische Universität München, Research Center for Nutrition and Food Science, Weihenstephaner Berg 3, 85350 Freising, Germany
Corresponding Author :Jakob Müller
Physiology Weihenstephan
Technische Universität München
Research Center for Nutrition and Food Science
Weihenstephaner Berg 3, 85350 Freising, Germany
 
Citation: Müller J, Pfaffl MW (2012) RNA Interference Off-target Screening using Principal Component Analysis. J Data Mining Genomics Proteomics 3:116. doi: 10.4172/2153-0602.1000116


RNA Interference Off-target Screening using Principal Component Analysis | Open Access | OMICS Publishing Group




Montag, 22. Juni 2015

RDML-Ninja and RDMLdb for standardized exchange of qPCR data.

RDML-Ninja and RDMLdb for standardized exchange of qPCR data.
Ruijter JM, Lefever S, Anckaert J, Hellemans J, Pfaffl MW, Benes V, Bustin SA, Vandesompele J, Untergasser A; and RDML consortium.
BMC Bioinformatics. 2015 16: 197


BACKGROUND: The universal qPCR data exchange file format RDML is today well accepted by the scientific community, part of the MIQE guidelines and implemented in many qPCR instruments. With the increased use of RDML new challenges emerge. The flexibility of the RDML format resulted in some implementations that did not meet the expectations of the consortium in the level of support or the use of elements.

RESULTS: In the current RDML version 1.2 the description of the elements was sharpened. The open source editor RDML-Ninja was released (http://sourceforge.net/projects/qpcr-ninja/ ). RDML-Ninja allows to visualize, edit and validate RDML files and thus clarifies the use of RDML elements. Furthermore RDML-Ninja serves as reference implementation for RDML and enables migration between RDML versions independent of the instrument software. The database RDMLdb will serve as an online repository for RDML files and facilitate the exchange of RDML data ( http://www.rdmldb.org ). Authors can upload their RDML files and reference them in publications by the unique identifier provided by RDMLdb. The MIQE guidelines propose a rich set of information required to document each qPCR run. RDML provides the vehicle to store and maintain this information and current development aims at further integration of MIQE requirements into the RDML format.

CONCLUSIONS: The editor RDML-Ninja and the database RDMLdb enable scientists to evaluate and exchange qPCR data in the instrument-independent RDML format. We are confident that this infrastructure will build the foundation for standardized qPCR data exchange among scientists, research groups, and during publication.


Dienstag, 12. Mai 2015

MIQE & qPCR iBook (1st edition)

MIQE & qPCR iBook

How to apply the MIQE guidelines - a visual, interactive and practical qPCR guide

1st edition by Afif M. Abdel Nour & Michael W. Pfaffl
ISBN 9783000488061
Free download on iTunes

Throughout the past 30 years Polymerase Chain Reaction (PCR) has proven to be the most powerful technique in a scientist toolbox. Only few techniques had a comparable success story like PCR. This iBook will guide you in better practicing in your laboratory thanks to the MIQE guideline.


More qPCR books and iBooks  http://qPCRbooks.gene-quantification.info

Montag, 11. Mai 2015

Advances in Oncology & Symposium on Molecular Medicine


20th World Congress on Advances in Oncology &
18th International Symposium on Molecular Medicine
October 8-10, 2015
Metropolitan Hotel, Athens, Greece
http://www.spandidos-publications.com/pages/conference



Pre-Congress qPCVR Workshops
in cooperation with the TATAA Biocenter, Sweden
6-7 October 2015
‘MIQE - How to get good-quality control in qPCR’ (2-day workshop by Dr. Kristina Lind)
‘Discovery and Validation of Expression Biomarkers — Experimental Design and Data Analysis’ (2-day workshop by Prof. Mikael Kubista)

Industrial Exhibition
In parallel to the scientific symposium of 2015, an Industrial Exhibition will take place, at which international companies, leaders in the fields of Oncology and Molecular Medicine, will present their latest technologies and services in clinical diagnostics. If you are interested in joining the exhibition

Contact Details

Metropolitan Hotel, Athens, Greece