Join our MicroGenomics 2021 Webinar Series

Oct. 23rd, Nov. 9th, Nov. 23rd, and Nov. 30th

15.00-17.30 CET

Participation is free of charge but your registration is mandatory

=> https://microgenomics.symposium.inrae.fr/2021-webinar-series

The International Symposium on Microgenomics 2021 in Florence, Italy

3rd International Symposium on Microgenomics

September 30 - October 1, 2021 in Florence, Italy

Today's challenge: understanding and analysing molecular targets within a given cell type in the tissue environment or liquid biopsies - isolating cells, extracellular vesicles (EV) or molecules - working on very small quantities of biological material. The aim of the symposium is to bring internationally renowned experts in the field together and offer an overview of the present knowledge for obtaining high quality cells, extracellular vesicles (EV) or molecules whether in plants, animals or humans.

Visit our web site for more information: https://microgenomics.symposium.inrae.fr/

Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR

Relative Expression Software Tool (REST©) for group wise comparison

and statistical analysis of relative expression results in real-time PCR

Michael W. Pfaffl, Graham W. Horgan & Leo Dempfle (2002) 

Nucleic Acids Research 2002 30(9): E36

http://www.gene-quantification.de/pfaffl-et-al-nar-2002.pdf

Real-time reverse transcription followed by polymerase chain reaction (RT–PCR) is the most suitable method for the detection and quantification of mRNA. It offers high sensitivity, good reproducibility and a wide quantification range. Today, relative expression is increasingly used, where the expression of a target gene is standardised by a non-regulated reference gene. Several mathematical algorithms have been developed to compute an expression ratio, based on real-time PCR efficiency and the crossing point deviation of an unknown sample versus a control. But all published equations and available models for the calculation of relative expression ratio allow only for the determination of a single transcription difference between one control and one sample. Therefore a new software tool was established, named REST© (relative expression software tool), which compares two groups, with up to 16 data points in a sample and 16 in a control group, for reference and up to four target genes. The mathematical model used is based on the PCR efficiencies and the mean crossing point deviation between the sample and control group. Subsequently, the expression ratio results of the four investigated transcripts are tested for significance by a randomisation test. Herein, development and application of REST© is explained and the usefulness of relative expression in real-time PCR using REST© is discussed. The latest software version of REST© and examples for the correct use can be downloaded at http://relative.gene-quantification.info/

Virtual Symposium -- ‪Diagnosing SARS-CoV-2 with qPCR‬

 
‪Diagnosing SARS-CoV-2 with qPCR‬

This virtual symposium, brought to you by The Scientist, will explore the limitations and challenges of current SARS-CoV-2 qPCR-based testing approaches, their root causes, and what can be learned for the future.
Tuesday, September 8, 2020
11:00 AM - 2:00 PM, Eastern Standard Time
https://www.the-scientist.com/sponsored-webinars/diagnosing-sars-cov-2-with-qpcr-symposium-67797

Stephen A. Bustin, PhD
Professor of Molecular Medicine
Faculty of Health, Education, Medicine and Social Care
Anglia Ruskin University

Jim F. Huggett, PhD
Senior Lecturer, Analytical Microbiology
School of Biosciences and Medicine
University of Surrey

Mikael Kubista, PhD
Head, Institute of Biotechnology
Czech Academy of Sciences
Founder and Chairman of the Board
TATAA Biocenter

Jo Vandesompele, PhD
Professor
Department of Biomolecular Medicine
Faculty of Medicine and Health Sciences
Ghent University

Michael W. Pfaffl, PhD
Professor of Molecular Physiology
Animal Physiology & Immunology
School of Life Sciences
Technical University of Munich

‪#qPCR #SARS_CoV_2 #MIQE

Digital MIQE Guidelines Update -- Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020

‪Read our latest dMIQE2020 update in Clinical Chemistry

Digital MIQE Guidelines Update -- Minimum Information

for Publication of Quantitative Digital PCR Experiments for 2020

https://academic.oup.com/clinchem/article/66/8/1012/5880117

‪#dPCR #MIQE #dMIQE2020‬ #ddPCR