Mittwoch, 24. Juni 2009

MIQE - qPCR Assay Quality Assessment

The MIQE guidelines: minimum information for publication of quantitativereal-time PCR experiments.
Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT. (
PDF)

Clin Chem. 2009 55(4): 611-622
http://www.rdml.org/miqe
http://miqe.gene-quantification.info
MIQE - media & press review

BACKGROUND: Currently, a lack of consensus exists on how best to perform andinterpret quantitative real-time PCR (qPCR) experiments. The problem isexacerbated by a lack of sufficient experimental detail in many publications,which impedes a reader's ability to evaluate critically the quality of theresults presented or to repeat the experiments.
CONTENT: The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelinestarget the reliability of results to help ensure the integrity of the scientific literature, promote consistency between laboratories, and increase experimentaltransparency. MIQE is a set of guidelines that describe the minimum informationnecessary for evaluating qPCR experiments. Included is a checklist to accompanythe initial submission of a manuscript to the publisher. By providing allrelevant experimental conditions and assay characteristics, reviewers can assess the validity of the protocols used. Full disclosure of all reagents, sequences,and analysis methods is necessary to enable other investigators to reproduceresults. MIQE details should be published either in abbreviated form or as anonline supplement.
SUMMARY: Following these guidelines will encourage betterexperimental practice, allowing more reliable and unequivocal interpretation of qPCR results.

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