Mittwoch, 16. Juni 2010

MIQE Press Review

MIQE PRESS REVIEW
http://miqe-press.gene-quantification.info/

Efforts to standardize qPCR data meets mixed reviews
05/25/2010Uduak Grace Thomas - BioTechniques
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Routine lab method's accuracy called into question
Catherine Shaffer - Nature Medicine Vol 16, page 349 (2010)
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The MIQE Guidelines Uncloaked - Speaker: Greg Shipley
8 June 2010 - The MIQE (Minimum Information for Publication of Quantitative Real-Time PCR Experiments) guidelines have been presented to serve as a practical guide for authors when publishing experimental data based on real-time qPCR. Each item is presented in tabular form as a checklist within the MIQE manuscript. However, this format has left little room for explanation of precisely what is expected from the items listed and no information on how one might go about assimilating the information requested. This presentation presents an expanded explanation of the guideline items with commentary on how those requirements might be met prior to publication.
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High-Impact Journals, Large Vendors Contributing to Lack of Quality Control for qPCR Data Publication
June 03, 2010 - By Ben Butkus
GÖTEBORG, Sweden – Quality control for publication of quantitative PCR data is still severely lacking – and, in the case of high-profile scientific journals such as Nature and Science, it is "absolutely appalling and scandalous" – according to Stephen Bustin, one of the scientists leading the drive to adopt a set of standards intended to guide high-quality and reproducible qPCR experiments within the field......
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Following MIQE Recommendations - EMBL Heidelberg, Germany
Monday 5 July - Friday 9 July 2010
Since the early descriptions of the use of quantitative Real Time PCR, the technique has been adopted in almost every aspect of life science research and is increasingly used for clinical analysis. Over time protocols and strategies have been tried and tested, amended and developed such that there are currently several different approaches. Protocol variations are evident at each step of the RT-qPCR process, from sample acquisition to data analysis (e.g. sample QC, experimental design, assay design and validation, normalisation, biostatistical interpretation, reporting, etc). It is now apparent that these adaptations may result in differences in the final biological conclusion of the study.
This workshop is based upon the MIQE guidelines. Each step of the RT-qPCR process will be discussed and protocol variations illustrated practically. The student will be instructed in best practice and acceptable alternative strategies.
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A Practical Guide to Publishing RT-qPCR Data That Conform to the MIQE …
In an effort to assist the scientific com- munity in producing consistent, high- quality data from qPCR experiments, the minimum information for publication of quantitative real-time PCR experiments .
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Real-time PCR on SciTopics
UPDATE on 21 January 2010 by Prof Stephen Bustin
Category: Biochemistry, Genetics and Molecular BiologyGuidelines for minimum information required for publication of qPCR data have now been published in Clinical Chemistry
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qPCR Assay Quality Assessment on SciTopics
UPDATE on 21 January 2010 by Prof Stephen Bustin
Category: Biochemistry, Genetics and Molecular BiologyGuidelines for minimum information required for publication of qPCR data have now been published in Clinical Chemistry
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MIQE Guidelines 'Slowly Filtering Through' PCR Community Despite Lack of Journal Enforcement
December 31, 2009 - Although the guidelines are beginning to catch on among researchers and vendors, they appear to have made little or no impact on the quality of the published literature over the last year.
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MIQE PRESS REVIEW
http://miqe-press.gene-quantification.info/

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