In search of better real-timePCR data

http://www.dddmag.com/article-In-Search-of-Better-Real-Time-PCR-10111.aspx

By Richard Kurtz, In Drug Discovery & Development - October 01, 2011

Real-time quantitative PCR (qPCR) has become the industry standard for the detection and quantification of nucleic acids. However, the lack of consensus among researchers on how to best perform and interpret qPCR experiments is a major hurdle for advancing the technology. This problem is exacerbated when insufficient experimental detail is given in published work, impeding the ability of others to accurately evaluate or replicate reported results.