Thursday 13 September 2012

MIQE Guidelines: A Roadmap for Proper qPCR Experimental Design and Reporting

MIQE Guidelines: A Roadmap for Proper qPCR Experimental Design and Reporting
Presented by Professor Stephen Bustin

Tuesday, September 25th. 2:00 pm
Tuesday, September 25th. 8:00 pm
* Times are in your local timezone.

Quantitative real-time PCR (qPCR) is today’s most widely used molecular technology, and continuous improvements to chemistries, enzymes, master mixes, and instruments have led to an increasingly reliable assay that virtually guarantees results. However, the same improvements that make it easy to generate qPCR data often conceal experimental practices that have resulted in the regular publication of data that are inconsistent, inaccurate and, sometimes, simply wrong. Incomplete reporting of experimental detail further confounds assessment of qPCR data validity, calling into question scientific conclusions that serve as a basis for further basic research and diagnostic applications. 

Learn how to:
- Design, evaluate, and report your qPCR experimental data according to the MIQE Guidelines.
- Enable the reproducibility of your experiment.

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