Discussion board for any problem in quantitative real-time PCR.
BLOG of www.Gene-Quantification.info
- the reference in real time PCR, the academic and industrial information platform. The GQ pages describe and summarize all technical aspects involved in quantitative gene expression analysis using real-time qPCR, digital PCR & RT-qPCR.
The analysis of transcripts in minute samples is often used to increase the knowledge in areas such as cancer development, plant physiology, neurodegenerative disorders of developmental biology. A very precise sampling crucial is crucial for the success of the approach. Depending on the nature of the sample and the goal of the experiment, there are different ways of sampling the probes. This course will present flow cytometry and laser capture microdissection as methods to obtain single cells for the subsequent gene expression analysis. The need for quantitative single-cell mRNA analysis is given by the inability of conventional RNA methods, to differentiate mRNA abundance of such limited samples. Another crucial step is data analysis, which constitutes another strong component of the course.
This course targets scientists who are entering the field of single cell analysis and want to learn about different preparation methods and gene expression profiling. The participants will learn about the complete workflow and gain understanding about the possibilities and limitations of the approach. The course will cover lectures and hands-on sessions by experts in the fields and is aimed for PhD students, postdoctoral fellows or other scientists with a solid qPCR and molecular biology background.