Mittwoch, 30. Oktober 2013

Nature Methods -- The need for transparency and good practices in the qPCR literature

NATURE METHODS | COMMENTARY
The need for transparency and good practices in the qPCR literature
Published online 30 October 2013

Stephen A Bustin, Vladimir Benes, Jeremy Garson, Jan Hellemans, Jim Huggett, Mikael Kubista, Reinhold Mueller, Tania Nolan, Michael W Pfaffl, Gregory Shipley, Carl T Wittwer, et al.
Nature Methods 10, 1063–1067 (2013)
http://www.nature.com/nmeth/journal/v10/n11/full/nmeth.2697.html

Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.

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