13 Jan 2014 by Sean Taylor, Bio-Rad Field Application Specialist
Scientists and journals have been slow to adopt the Minimum Information for the Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines that were established in 2009 to bolster the reliability of real-time PCR (qPCR) and reverse transcription qPCR (RT-qPCR) data. To help boost adoption, Bio-Rad scientists published a brief and practical guide that concisely summarizes the key steps required to produce high-quality, reproducible data for labs conducting RT-qPCR experiments.
We spoke co-author of the guide, Sean Taylor, to understand more the importance of MIQE, the low adoption rates and what the future may hold.
More info here http://MIQE.gene-quantification.info/