Donnerstag, 27. Februar 2014

Careful strategizing helps make PCR sensitive enough for the most challenging experiments

PCR: paths to sensitivity
by Vivien Marx
TECHNOLOGY FEATURE Nature Methods 11, 241–245 (2014) doi:10.1038/nmeth.2849
Published online 27 February 2014

Careful strategizing helps make PCR sensitive enough for the most challenging experiments.

Scientists may think PCR is simple, says Ghent University researcher Jo Vandesompele, “but it's not.” He splits his time between his own research and Biogazelle, a university spin-out that he cofounded and of which he is CEO. His team developed qPCR analysis software called qbase+, and he has a statistical algorithm and software for dPCR in the works. He consults and sponsors training in PCR techniques to help a lab or a company department standardize its practices and to strategize about experiments. Courses have focused on qPCR best practices, and this year he is adding dPCR.
http://www.nature.com/nmeth/journal/v11/n3/full/nmeth.2849.html

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