Discussion board for any problem in quantitative real-time PCR.
BLOG of www.Gene-Quantification.info
- the reference in real time PCR, the academic and industrial information platform. The GQ pages describe and summarize all technical aspects involved in quantitative gene expression analysis using real-time qPCR, digital PCR & RT-qPCR.
Prime time for qPCR – raising the quality bar Mikael Kubista, TATAA Biocenter, SwedenQuantitative Real-Time Polymerase Chain Reaction, better known as qPCR, is the most sensitive and specific technique we have for the detection of nucleic acids. Even though it has been around for more than 30 years and is preferred in research applications, it has still to win broad acceptance in routine. Main hurdles are the lack of guidelines, standards, quality controls, and even proper methods to evaluate the diagnostic results. This is now rapidly changing.
PCR to diagnose infectious disease Theo P. Sloots and Michael D. Nissen, Queensland Children’s Medical Research Institute, AustraliaEffective clinical management and treatment of patients with infectious disease relies on the rapid and accurate identification of the causative agent. Yet, until recently, diagnosticians used relatively insensitive methods to detect the infectious agent in clinical specimens, namely microscopy, histology, isolation and culture of pathogens and by measuring the serologic response of the host.
qPCR Roundtable Moderated by Prof. Dr. Michael W. Pfaffl, Department of Physiology, Life Science Centre Weihenstephan, Technical University of Munich