Nov 13, 2014 via GenomeWeb
Though reverse transcription of RNA to cDNA is an essential first step for a growing number of genomics applications, researchers have long known of problems associated with RT and its effects on study results. Now, a new study has examined some of those issues and has provided a workflow and guidelines for researchers publishing RT-based data.
The study, published in Clinical Chemistry late last month, was undertaken by some of the authors of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments, or MIQE, guidelines, which debuted in 2009. It compared measures of different mRNA targets using six commercially available RT enzymes and varying sample concentrations of differing qualities. Results showed the variability was "sufficiently large to call into question the validity of many published data that rely on quantification of cDNA."
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