An Interview with MIQE Guru and qPCR Expert Stephen Bustin

seen on - European Biotechnologist
Stephen Bustin obtained his Ph.D. from Trinity College, University of Dublin in molecular genetics in 1983. He is currently Professor of Molecular Science at Barts and the London School of Medicine and Dentistry where he aims to apply his research in a more translational setting by doing basic research and clinical practice in colorectal cancer. Bustin’s priority research aim is to translate his lab’s distinctive approach for predicting metastatic behaviour of colorectal cancer into a practical and robust assay for cancer prognosis that is not just more accurate than anatomically-based staging but can identify candidate therapeutic targets in metastatic colorectal cancers.
In a seminal paper published in Clinical Chemistry, Stephen Bustin et al. published a new set of guidelines that describe the minimum information necessary for evaluation of quantitative real-time polymerase chain reaction experiments. Following the Minimum Information for publication of Quantitative real-time PCR Experiments (MIQE) guidelines will encourage better experimental practice, allowing more reliable and unequivocal interpretation of quantitative PCR results.
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