Mittwoch, 29. September 2010

Join our microRNA qPCR application workshop in November


Theoretical and practical parts about small silencing RNA (miRNA, siRNA) with focus on quantitative microRNA analysis. For experienced users in miRNA and PCR.
Language: In English
Date: 8-9 November 2010
Location: BioEPS GmbH, Lise-Meitner-Strasse 30, 85354 Freising, Germany (very close to Munich airport MUC)
Link => http://site.bioeps.com/index.php?option=com_seminar&Itemid=6
Day 1: Introduction, microRNA and RNA Quality
- Introduction siRNA, microRNA, piRNA, RNAi: biogenesis, action and applications
- RNA Isolation: setting up a miRNA based experiment
- microRNA Quantity and Quality Control: methods and applications
- RT-qPCR: priciples of RT and qPCR, difference between enzymes and primers
- Optimization strategies for miRNA
Practical Part: real-time miRNA-qPCR Experiment
Day 2: Normalization and Quantification
- Data analysis: principles of qPCR softwares, algorithms and melt curve analysis
- Quantification strategies: absolute and relative quantification strategies
- Normalization with miRNA: how to choose a good reference
Practical Part: real-time miRNA-qPCR Experiment

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