Theoretical and practical parts about small silencing RNA (miRNA, siRNA) with focus on quantitative microRNA analysis. For experienced users in miRNA and PCR.
Language: In English
Language: In English
Date: 8-9 November 2010
Location: BioEPS GmbH, Lise-Meitner-Strasse 30, 85354 Freising, Germany (very close to Munich airport MUC)
Link => http://site.bioeps.com/index.php?option=com_seminar&Itemid=6
Day 1: Introduction, microRNA and RNA Quality
- Introduction siRNA, microRNA, piRNA, RNAi: biogenesis, action and applications
- RNA Isolation: setting up a miRNA based experiment
- microRNA Quantity and Quality Control: methods and applications
- RT-qPCR: priciples of RT and qPCR, difference between enzymes and primers
- Optimization strategies for miRNA
Practical Part: real-time miRNA-qPCR Experiment
Day 2: Normalization and Quantification
- Data analysis: principles of qPCR softwares, algorithms and melt curve analysis
- Quantification strategies: absolute and relative quantification strategies
- Normalization with miRNA: how to choose a good reference
Practical Part: real-time miRNA-qPCR Experiment
Location: BioEPS GmbH, Lise-Meitner-Strasse 30, 85354 Freising, Germany (very close to Munich airport MUC)
Link => http://site.bioeps.com/index.php?option=com_seminar&Itemid=6
Day 1: Introduction, microRNA and RNA Quality
- Introduction siRNA, microRNA, piRNA, RNAi: biogenesis, action and applications
- RNA Isolation: setting up a miRNA based experiment
- microRNA Quantity and Quality Control: methods and applications
- RT-qPCR: priciples of RT and qPCR, difference between enzymes and primers
- Optimization strategies for miRNA
Practical Part: real-time miRNA-qPCR Experiment
Day 2: Normalization and Quantification
- Data analysis: principles of qPCR softwares, algorithms and melt curve analysis
- Quantification strategies: absolute and relative quantification strategies
- Normalization with miRNA: how to choose a good reference
Practical Part: real-time miRNA-qPCR Experiment
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